BIOLOGICAL INPUTS FOR SUSTAINABLE AGRICULTURE & ENVIRONMENT
BIO-FOLIARCARE FOR PESTS
- Bio-Foliar Care consisting of mixer of Biopesticides such as Bacillus thuringiensis var kurstaki, Beauveria bassiana, Metarhizium anisopliae, Verticillium lecanii and Paecilomyces fumosoroseus. Bio-Foliar Care would control most of pathogenic pests and insects.
Bacillus thuringiensis var kurstaki
- Bacillus thuringiensis var kurstaki is an entomopathogenic bacterium which produces parasporal crystalline protein inclusion, when ingested by a susceptible insect, the crystalline protein damages the gut lining, leading to gut paralysis. The affected insects stop feeding and die from the combined effects of starvation and tissue damage.
Beauveria bassiana
- Beauveria bassiana is a naturally occurring entomopathogenic fungus. The spores of this fungus when comes in contact with the cuticle (skin) of the target insect, the spores will germinate and grow on the cuticle and enter into the inner body of the infected insect. The fungus proliferates throughout the insect’s body, draining the insect’s nutrients, eventually killing it in about 48-72 hours after infection.
Metarhizium anisopliae
- Metarhizium anisopliae is an entomopathogenic fungus that infects the Root weevils, plant hoppers, Japanese beetle, Black vine weevil, Spittlebug and white grubs. The spores of this fungus when comes in contact with the cuticle (skin) of the target insect, the spores will germinate and grow, they then penetrate the exoskeleton of the insect and grow very rapidly inside the insect and cause the insect to die. Other insects that come in contact with infected insects also become infected with the fungus.
Verticillium lecanii
- Verticillium lecanii is an entomopathogenic fungus. The mycelium of this fungus produces a cyclodepsipeptide toxin called bassianolide and other insecticidal toxins such as dipicolinic acid, which infect aphids, whiteflies, rust fungi, scale insects and lead to death the host.
Paecilomyces fumosoroseus
- Paecilomyces fumosoroseus, is considered a very promising biological pesticide due to its extensive host range which includes insects in over 25 different families, including the diamondback moth (Plutella xyllostella), Russian wheat aphid (Diuraphis noxia), silver leaf whitefly (Bemisia argentifolii) and wide range of mites (Spotted Spider Mite, Red Mite, Brown Mite, and Rust Mite).
Target Crops
- Orchards, Flowers, Ornamentals, Nurseries, Vegetables, Maize, Rice, Cotton, Cole crops and Plantation crops.
Powder Formulation
- Recommended Dosage
- Foliar spray: Dilute 10 gms per litre of water, the spray volume depends on the crop canopy.
- Product composition
- Live culture : 1x10^9 CFU/gm.
- Carrier material : Talc powder.
Liquid Formulation
- Recommended Dosage
- Foliar Spray: 5 ml per litre of water, the spray volume depends on the crop canopy.
- Product composition
- Live Culture : 1x10^10 CFU/ml
- Carrier material : Liquid base (100% water soluble)
BIO-NUTRICARE
- Bio-Nutri Care consisting of Nitrogen fixers (Azospirillum sp., Rhizobium sp.), Phosphate solubilizers (Bacillus sp. Pseudomonas sp.), Potash mobilizer (Fraturia sp.) and Vesicular Arbuscular Mycorrhizae (VAM). The combination of these microbes would fulfill the plants nutrient requirements.
Nitrogen fixers
- Nitrogen fixers (Azospirillum sp. Azotobacter sp. and Rhizobium sp.), converts atmospheric nitrogen gas into plant usable ammonium forms and fulfills of plants nitrogen requirements. Nitrogen fixers produce plant growth promoting substances viz, vitamins, auxins and gibberellins which influences plant growth to a large extent. Nitrogen fixer enhances seed germination, early seedling vigor and increases the crop yield.
Phosphate solubilizers
- Phosphate solubilizers (Bacillus megaterium var. phosphaticum and Pseudomonas striata) dissolve fixed tricalcium phosphate into plant utilizable monocalcium phosphate form. Phosphate solubilizers produce organic acids (viz, citric acid, succinic acid, lactic acid, etc.), hormones (viz, indole acetic acid, gibberellins etc.) and enzymes (viz, phytase, nuclease, lecithinase etc.) that help, in solubilisation of insoluble phosphates into plant utilizable form. Phosphate solubilizers improve plants growth and increase the crop yield.
Potash mobilizer
- The microbe, Frateuria aurentia is a beneficial bacterium capable of mobilizing soil available Potash into near the root zone of plants. It works well in all types of soil especially, low K content soil. Use of such bacteria in powder form can increase the availability of more potash in usable form to the plants.
Vesicular Arbuscular Mycorrhizae (VAM)
- Mycorrhizae, mobilizing major nutrients like, nitrogen, phosphorus and potassium and also certain micronutrients like zinc, calcium, iron, magnesium and manganese. Mycorrhizal inoculation enhanced absorption water, storage and movement back into plants enhances tolerance to drought. Mycorrhizae produce specific antibiotics, which immobilize and kill soil borne pathogens. Mycorrhizal inoculation reduces the chemical fertilizer application and enhances the crop yield.
Target Crops
- Cereals - rice, wheat, millet, Vegetables - Bean, Black eyed Pea, cabbage, cauliflower, lettuce, Carrot, Cucumber, Eggplant, Melon, Mustard, Onion, Parsnip, Pea, Pepper, Potato, etc.,
Powder formulation
- Recommended Dosage
- Soil application: 2 kg /acre along with any organic fertilizer (without pathogenic contaminants).
- Seed treatment: @ 4-5 gm per kg of seeds as per standard wet treatment.
- Seedling treatment: @ 100 g/l prior to planting.
Liquid Formulation
- Recommended Dosage
- Soil application: 1 litre /acre
- Seed treatment: @ 10 ml per kg of seeds as per standard wet treatment.
- Seedling treatment: @ 10 ml/litre of water prior to planting.
BIO-ROOTCARE
- Bio-Root Care consisting of mixer Biocontrol agents such as Pseudomonas fluorescens, Trichoderma viride, Trichoderma harzianum, Bacillus subtilis, Paecilomyces lilacinus. The combination these biocontrol agents would control most of soil borne pathogens.
Pseudomonas fluorescens
- Pseudomonas fluorescens as non-pathogenic saprophytes that colonize soil, water and plant surface environments. Pseudomonas fluorescens suppress plant diseases by production of number of secondary metabolites including antibiotics, siderophores and hydrogen cyanide. This microbe has the unique ability to enter the plant vascular system, reach the various parts of the plant system and act as a systemic bio-control agent against various fungal and bacterial diseases.
Bacillus subtilis
- Bacillus subtilis is a spore forming omnipotent bacteria which, when applied to the seeds or growing plants, it colonizes on the developing root system of the plants. Bacillus subtilis can able to multiply even in poor soil organic content and compete with and thereby suppress the growth of various pathogenic fungal organisms such as Rhizoctonia spp., Fusarium spp., Aspergillus spp., and others.
Trichoderma viride / harzianum
- Trichoderma spp. grows tropically toward hyphae of other pathogenic fungi, coil about them in a lectin-mediated reaction, and degrade cell walls and kill the pathogenic fungi by secreting of extracellular enzymes viz. Chitinase and Peptaibols. This process (mycoparastitism) limits growth and activity of plant pathogenic fungi.
Paecilomyces lilacinus
- Paecilomyces lilacinus is a naturally occurring fungus found in many kinds of agricultural soils. Paecilomyces lilacinus is widely used to control various plant pathogenic nematodes viz. Root knot nematodes, Burrowing nematodes, Cyst nematodes, Root lesion nematodes, Reniform Nematodes and False Root knot Nematodes.
Target Crops
- Eggplant, Potato, Chilli, Tomatoes, Cucumbers, Flowers, Orchards, Vineyards, Ornamental plants, lawns and nurseries etc.
Powder formulation
- Recommended Dosage
- Soil application: 2 kg /acre
- Seed treatment: @ 4-5 gm per kg of seeds as per standard wet treatment.
- Seedling treatment: @ 100 g/l prior to planting.
Product composition
- Live Culture : 1x10^10 CFU/gm
- Carrier material : Talc powder
Liquid Formulation
- Recommended Dosage
- Soil application: 1 litre /acre
- Seed treatment: @ 10 ml per kg of seeds as per standard wet treatment.
Product composition
- Live Culture : 1x10^11 CFU/ml
- Carrier material : Liquid base (100% water soluble)
BIO-FOLIARCARE FOR DISEASES
- Bio-Foliar Care for Diseases consisting of Bacillus subtilis, Bacillus pumilus, Pseudomonas fluorescens and Streptomyces lydicus, suitable to control wide range of Bacterial and Fungal Diseases in plants.
Bacillus subtilis and Bacillus pumilus
- Bacillus subtilis and Bacillus pumilus are spore forming bacteria, it can able to survive on foliage and compete with and thereby suppress the bacterial and fungal diseases.
Pseudomonas fluorescens
- Pseudomonas fluorescens suppress plant diseases by production of number of secondary metabolites including antibiotics, siderophores and hydrogen cyanide. This microbe has the unique ability to enter the plant vascular system, reach the various parts of the plant system and act as a systemic bio-control agent against various fungal and bacterial diseases.
Streptomyces lydicus
- Streptomyces lydicus is a species from Actinomycetes group (undifferentiated fungal and bacterial species), produces variety of secondary metabolites, antibiotics (e.g., Streptomycin, Neomycin and Chloramphenicol) and enzymes. Streptomyces lydicus has the unique ability to enter the plant vascular system, reach the various parts of the plant system and act as a systemic bio-control agent against various fungal and bacterial diseases.
Target Crops
- Orchards, Flowers, Ornamentals, Nurseries, Vegetables, Maize, Rice, Cotton, Cole crops and Plantation crops.
Powder Formulation
- Recommended Dosage
- Foliar spray: Dilute 10 gms per litre of water, the spray volume depends on the crop canopy.
- Product composition
- Live culture : 1x10^10 CFU/gm.
- Carrier material : Talc powder.
Liquid Formulation
- Recommended Dosage
- Foliar Spray: 5 ml per litre of water, the spray volume depends on the crop canopy.
- Product composition
- Live Culture : 1x10^11 CFU/ml
- Carrier material : Liquid base (100% water soluble)
LAWN CARE
- Lawn Care is a multifaceted consortium of beneficial enzymes with live cells from Bacillus subtilis, Streptomyces lydicus, Bacillus firmis, Pseudomonas fluorescens, Trichoderma viride, Azotobacter chroococcum as well from Endomycorrhizae’s such as Glomus intraradices, Glomus clarum, Glomus aggregatum and Glomus fasciculatum, useful to nourish and protect the Lawn throughout the life cycle.
- Lawn Care microbes has the unique ability to enter the plant vascular system, reach the various parts of the plant system and deliver nitrogen by natural nitrogen fixation, plant growth hormones, essential amino acids and enzymes. The Lawn Care enzymes (Cellulose, Ligninase, Amylase, Pectinase etc.,), breakdown the fallen leaves and recycle it and supply back as enriched nutrient to lawn grass. Lawn Care act as a systemic bio-control agent against various fungal and bacterial diseases by production of number of secondary metabolites including antibiotics and siderophores.
Dosage and Application
- Dilute 1 litres of Lawn Care in 200 litres of water and spray for an acre (4000 M2) area and repeat at 6 weeks intervals.
- Lawn Care should not be mixed with chlorinated water.
Composition
- Mixer of enzymes and live microbial cells – 90%
- Liquid Base – 10%
SEWAGE CARE
- The increasing odour by biological and chemical wastes into the municipal waste treatment systems is the major concern to treat. Bio-augmentation of required microorganisms for sewage care is a new technology developed by the Dr. Rajan Laboratories in the name of Sewage Care.
Microbial Composition
- Bacillus sp.
- Chromatium sp.
- Nitrosomonas sp.
- Nitrobacter sp.
- Rhodobacter sp.
Advantages
- Decompose complex molecules into simpler molecules
- Transforms ammonia into nitrate
- Enhances the oxygen dissolving capacity of polluted sewage and thereby helps the mechanical process.
Powder formulation
- Dosage and application
- Apply 2 Kgs. /100M3of waste in weekly interval.
Product composition - Live culture : 1x10^9 CFU/gm.
- Carrier material : Talc powder.
Liquid formulation
- Dosage and application
1 lit. /100M3of waste in weekly interval.
Product composition
- Live Culture : 1x10^10 CFU/ml
- Carrier material : Liquid base
AQUA CARE
- Aqua care is consortium of non-pathogenic nitrifying bacteria, these bacteria converts / fixes gaseous Ammonia into Ammonium form. Bacteria of the genus Nitrosomonas are chemoautotrophs that take part in the decomposition of waste into nutritive organic fertilizer. They use ammonia as their electron source in a reaction known as nitrification:
NH4+ + ½ O2 + H2O --- NO2- + 2H2O + 2H+
- The organic N present in proteins, amines and urea is hydrolysed to ammonia and is then oxidised to nitrate by Nitrosomonas creating new cells with chemical form C5H7O2N as:
55NH4+ + 76O2 + 109HCO3--- C5H7O2N + 54NO2- + 57 H2O + 104H2CO3
- Following this, another genus of chemoautotrophs called Nitrobacter utilize nitrite as their energy source in a second stage of nitrification and covert to plant utilizable nitrate form the reaction could be generally represented as:
NO2- + ½ O2 ---- NO3-
- Powder formulation
- Dosage and application
- Apply 2 Kgs. /100M3of waste in weekly interval.
- Product composition
- Live culture : 1x10^10 CFU/gm.
- Carrier material : Talc powder.
- Liquid formulation
- Dosage and application
- 1 lit. /100M3of waste in weekly interval.
- Product composition
- Live Culture : 1x10^11 CFU/ml
- Carrier material : Liquid base (100% water soluble)
PROBIOTICS FOR POULTRY
- PROBIOTICS FOR POULTRY is a consortium of lactic acid producing bacteria and other beneficial microbes useful to prevent intestinal disorders in poultry. These microbes will secrete variety of enzymes viz. amylase, cellulase, and protease were improve absorption of nutrients in digestion.
Microbes and its Benefits
Aspergillus oryzae
- Secrets amylase and provides essential amino acids and vitamins and controls the solidities of feces.
Bacillus subtilis, B.amyloliquefaciens, B.licheniformis, B.pumilus
Secrets cellulase and protease.
Lactobacillus acidophilus and Enterococcus faecium
- Useful intestinal bacteria in poultry, serves to prevent intestinal disorder, prevents diarrhea and promotes overall growth.
Saccharomyces cerevisiae
- Maintains balance of intestinal microorganisms
Promotes absorption of nutrients from feed
Increase weight of livestock and provides growth factor.
Powder formulation
- Recommended Dosage
- 1 kg / 1000 kgs of feed material
Product composition
- Fungal spores and Bacterial cells : 1x109 CFU/gm.
- Carrier material : Talc powder.
Liquid formulation
- Recommended Dosage
- 1 litre per 1000 litre of drinking water
Product composition
- Fungal spores and Bacterial cells : 1x1010 CFU/ml.
- Carrier material : Liquid base (100% water soluble).
PROBIOTICS FOR AQUACULTURE
- PROBIOTICS FOR AQUACULTURE is a consortium of lactic acid producing bacteria and other beneficial microbes useful to prevent intestinal disorders in Aqua Farming. These microbes will secrete variety of enzymes viz. amylase, cellulase, and protease were improve absorption of nutrients in digestion.
Microbes and its Benefits
Aspergillus oryzae
- Secrets amylase and provides essential amino acids and vitamins and controls the solidities of feces.
Bacillus subtilis, B.amyloliquefaciens, B.licheniformis, B.pumilus
Secrets cellulase and protease.
Nitrosomonas sp. and Nitrobacter sp.
- Converts toxic ammonia in to nitrate and hydrogen sulphide in to sulphate, it helps to reduce Biological Oxygen Demand (BOD) and increase dissolved oxygen levels.
Saccharomyces cerevisiae
Maintains balance of intestinal microorganisms.
Powder formulation
- Recommended Dosage
- 1 kg / 1000 kgs of feed material
Product composition
- Fungal spores and Bacterial cells : 1x109 CFU/gm.
- Carrier material : Talc powder.
Liquid formulation
- Recommended Dosage
- 1 litre per 1000 litre of drinking water
Product composition
- Fungal spores and Bacterial cells : 1x1010 CFU/ml.
- Carrier material : Liquid base (100% water soluble).
BIODEGRADER
- Organic matter is a vital for maintaining soil fertility as it provides different plant nutrients and suitable environment for microbial population and improves physio-chemical properties of soil. But in its natural structure, without being decomposed, the organic matter contributes little to the improvement of soils and in crop production. The plant nutrients in these materials become available only through decomposition. Generally, under normal environment, it takes 3-8 months to get it decomposed while with addition of certain microorganisms, the time of decomposition can be shortened to 4-6 weeks.
- Biodegrader is consortium different microbes that activate and rapidly multiply in the manure composting pit with in 24 h after the application. The synergistic action of bio-augmentation and enzymatic catalysis breaks down even the toughest of animal and plant wastes into healthy, enriched, nutrient-balanced compost. Biodegrader produces large amounts of self-generating dissolved and free oxygen, which accomplishes aeration without turning the compost pit either manually or mechanically, thus saving both labor and energy.
Application Method and Dosage
- The most effective application method is to dilute 1 Kg or 500 ml (to decompose one metric ton of waste) of Biodegrader in 100 litres of 1% sugar solution (or) 1% molasses and spray layer by layer on the waste. Alternatively, a number of vertical holes can be made in the manure pit and diluted Biodegrader poured in it. Compost heaps should be covered with straw or plant-wastes to retain 40-50% moisture (Ambient temperature for decomposition). Composting would be completed by 30-45 days; it varies depend on ambient temperature, raw material and climatic conditions.
Powder Formulation
Recommended Dosage
- Spray or Pour: Dilute 1 kg in 100 litre of water and spray to decompose 1 ton of farm residues as recommended above.
- Product composition
- Live culture : 1x109 CFU/gm.
- Carrier material : Talc powder.
Liquid Formulation
Recommended Dosage
- Spray or Pour: Dilute 1 kg in 100 litre of water and spray to decompose 1 ton of farm residues as recommended above.
- Product composition
- Live Culture : 1x10^10 CFU/ml
- Carrier material : Liquid base (100% water soluble)
ODOUR CARE
- ODOUR CARE is a microbial blend of preselected, adapted microorganisms for use under microaerophilic, aerobic or anaerobic conditions. It is formulated for use in Septic tank, Sludge, Compost, Contaminated soils and wastewaters to suppress hydrogen sulfide odors and enhance biodegradation and contaminant removal where oxygen is of limited availability.
Advantages of using Odour Care
- To reduce the ammonia toxicity in urinals / septic tanks.
- To reduce the bad odor in garbage area.
- To reduce the high chemical usage for treating leachate in industrial area.
Powder Product composition
- Live Culture : 1x10^10 CFU/ml
- Carrier material : talc base
Dosage and application
- Sprinkle on the target sites.
Liquid Product composition
- Live Culture : 1x10^11 CFU/ml
- Carrier material : Liquid base
Dosage and application
- Dilute 1 litre of Odour Care in 200 litre of water, sprinkle or spray in target sites.
EFFLUENT CARE
- EFFLUENT CARE is useful for treatment of effluent from Agro-Industries, Pharmaceutical industries and Garbage dumping sites of small and medium size Community holdings. Bio-augmentation of required microorganisms for reduces B.O.D and C.O.D is a new technology developed by the Dr. Rajan Laboratories in the name of product B.O.D, C.O.D and odor remover.
Microbial Composition
- Saline resistant Nitrosomonas sp.
- Saline resistant Nitrobacter sp.
- Bacillus sp.
- Rhodopseudomonas sp.
- Thiobacillus sp.
- Pseudomonas sp.
Advantages
- The high and toxic levels of Nitrogen, Phosphorus, Carbon, BOD, COD, Sulphur etc
- can be treated.
- Reduces ammonia toxicity.
- Reduces volatile fatty acids and mal odours of sulphur.
Powder formulation
Dosage and application
- Apply 2 Kgs. /100M3of waste in weekly interval.
Product composition
- Live culture : 1x10^9 CFU/gm.
- Carrier material : Talc powder.